ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot. Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, native (non-denaturing, non-dissociating) PAGE, and western blotting for highly sensitive qualitative analysis.
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Description
Anti-Collagen Type IV has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Immunogen
Collagen Type IV from human and bovine placenta
Other Names
rabbit anti-Collagen Type IV antibody, Arresten antibody, Canstatin antibody, Collagen Of Basement Membrane Alpha 1 Chain antibody, Collagen alpha-1 (IV) chain, COL4A1
Hibiki Vincent Tanaka, Nathaniel Chuen Yin Ng, Zhan Yang Yu, Martin Miguel Casco-Robles, Fumiaki Maruo, Panagiotis A. Tsonis, Chikafumi Chiba. (2016) A developmentally regulated switch from stem cells to dedifferentiation for limb muscle regeneration in newts. Nature Communications vol 7, Article: 11069. doi:10.1038/ncomms11069.