Immunogen sequence: L17-N294. Specificity: Natural and recombinant mouse OPN. There is no detectable cross-reactivity with other relevant proteins.
Size
1 kit
Concentration
n/a
Applications
ELISA. Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse OPN standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. It is recommended that each mouse OPN standard solution and each sample be measured in duplicate.
Other Names
AccuSignal BNSP, Bone sialoprotein 1, Bone sialoprotein I, BSPI, Early T lymphocyte activation 1, ETA 1, MGC110940, Nephropontin, OPN, Osteopontin, OSTP_HUMAN, Secreted phosphoprotein 1, Secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1), Secreted phosphoprotein 1 (osteopontin bone sialoprotein), SPP-1, SPP1, Urinary stone protein, Uropontin