This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for cTn-I has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any cTn-I present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for cTn-I is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme
[TNNI3; TNNI3 protein; troponin I, cardiac muscle; cardiomyopathy, dilated 2A (autosomal recessive); troponin I type 3 (cardiac); TNNI3 protein; Troponin I type 3 (Cardiac); Troponin I, cardiac; Troponin I, cardiac variant]