Cayman's PPARγ FP-Based Ligand Screening Assay - Green provides a convenient fluorescence polarization (FP)-based single step assay for screening PPARγ ligands. In this assay, a ligand of PPARγ was conjugated to FITC and is used as the displacement probe. Agonists and antagonists of PPARγ will displace the fluorescent probe leading to a decrease in FP. The PPARγ FP-Based Ligand Screening Assay is a robust assay with a Z' of 0.81 and has a dynamic range of greater than 120 mP units. The assay has been validated using known agonists/ligands of PPARγ (Arachidonic Acid, Rosiglitazone, Troglitazone, etc.) with IC50 values ranging from nanomolar to millimolar concentrations.