This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with LTB4 . During the reaction, LTB4 in the sample or standard competes with a fixed amount of LTB4 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to LTB4 . Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is adde