Immunofluorescence: Assay Dependent, Immunoprecipitation: Assay Dependent, Western Blot: 1-3 μg/mL
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Description
TOP1 Monoclonal Antibody (24.5). Topoisomerases are nuclear enzymes involved in a variety of cellular activities such as chromosome condensation, DNA replication, transcription, recombination and segregation at mitosis. Human topoisomerase I is a 100kDa protein capable of relaxing positively and negatively supercoiled DNA by performing a transient single stranded nick which is then relegated at the end of the reaction. It has been shown that the enzyme is located in regions of the genome that are undergoing active RNA synthesis, where it probably reduces superhelical stresses in the DNA, enabling RNA polymerase to function properly. Both DNA topoisomerases I and II have been found to be targets of autoantibodies in the sera of patients with
Immunogen
n/a
Other Names
TOPI, DNA topoisomerase 1, type I DNA topoisomerase