Purity ~90% (SDS-PAGE). Hexokinase2 is one of four highly homologous hexokinase isoforms in mammalian cells. Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria. Source: Recombinant protein corresponding to aa1-546 from human Hexokinase2, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~104.5kD (937aa) Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Specific Activity: >40,000pmol/min/ug. Unit Definition: One unit will convert 1 pmoles of D-Glucose to D-Gluco