Hexokinase 1, Recombinant, Human (Hexokinase type I, Brain form hexokinase, HK1)
Description
Purity ~90% (SDS-PAGE). Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV This enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this hexokinase1 have been associated with hemolytic anemia due to hexokinase deficiency. Source: Recombinant protein corresponding to human Hexokinase 1, expressed in E. coli. Molecular Weight: ~104.6kD Biological Activity: Specific activity is 7-8units/ml obtained by measuring the increase of NADPH in absorbance at 340nm resulting from the reduction of NADP. AASequence: MGSSHHHHHH SSGLVPRGSH MIAAQLLAYY FTELKDDQVK KIDKYLYAMR