Highly Purified (~95%). Hexokinase catalyzes the first step of several metabolic pathways by converting D-hexose to D-hexose-6-P. Hexokinase is an allosteric enzyme inhibited by its products glucose-6-phosphate. At least 4 related hexokinase isoforms (HXKI-III; HXK-IV also known as Glucokinase) have been cloned and characterized. Hexokinases (~100kD for HXK1-III; HXKIV lacks the N-terminal domains and is ~50kD) are outer mitochondrial membrane proteins. The N-terminus, containing the mitochondrial target sequence, and the C-terminal has high sequence homology among various isoforms. The catalytic activity is associated with the C-terminus and other regulatory functions are controlled by the N-terminus. HXKII is located on chromosome 2. HXKII is the predominant isozyme that is expressed in young red cell, the skeletal muscle, adipose tissue, and reticulocytes. It is regulated by insulin. Defects in HXKII are a cause of monogenic autosomal dominant non-insulin-dependent diabetes mellitus
Size
10ug
Concentration
n/a
Applications
ELISA WB
Other Names
Hexokinase-2, Recombinant, Human, His-Tag (EC 2.7.1.1, HK2, Hexokinase Type II, HK II, Muscle Form Hexokinase, HXK2, DKFZp686M1669)