Hexokinase Type III, Recombinant, Human (HK3, HKIII)
Description
Purity ≥95% by SDS PAGE. Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase3 lacks the hydrophobic N-terminal sequence critical for targeting to mitochondria. Hexpkinase3 may have anabolic functions, providing H6P for glycogen or lipid synthesis. Recombinant human Hexokinase3, fused to His tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. Molecular Weight : 101.1kD (943 amino acids) Sequences: MGSSHHHHHH/ SSGLVPRGSH/ MDSIGSSGLR/ QGEETLSCSE/ EGLPGPSDSS/ ELVQECLQQF/ KVTRAQLQQI/ QASLLGSMEQ/ ALRGQASPAP/ AVRMLPTYVG/ STPHGTEQGD/ FVVLELGATG/ ASLRVLWVTL/ TGIEGHRVEP/ RSQEFVIPQE/ VMLGAGQQLF/ DFAAHCLSEF/ LDAQPVNKQG/ LQLGFSFSFP/ CHQTGLDRST/