Purity ≥90% by SDS PAGE. Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria. Recombinant human Hexokinase2, fused to His tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. Specific Activity: 3-4 units/ml obtained by measuring the increase of NADPH in absorbance at 340nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 25°C. Activity Assay: 1. Prepare a 2.57ml reaction mixture into a suitable co