ELISA, Immunohistochemistry, Western Blot. This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 170 kDa in size corresponding to phosphorylated EGFR protein by western blotting in the appropriate cell lysate or extract. Less than 5.0% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pY1197 of EGFR protein.
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Description
This affinity-purified antibody is directed against the phosphorylated form of human EGFR protein at the pY1197 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human EGFR pY1197 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human EGFR is minimal by ELISA. A BLAST analysis was used to suggest cross reactivity with phosphorylated EGFR from human, mouse and rat sources. Reactivity of this antibody with EGFR from other species is unknown.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1189-1199 of human EGFR protein.