ELISA, Immunohistochemistry, IF Microscopy, Western Blot. Beta-Gal Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunofluorescence microscopy, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4% paraformaldehyde fixed tissue for most immunohistochemical analysis. A 1:1,500 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ. In this particular experiment, tissue was perfused with 4% paraformaldehyde and cryostat-cut (35 μm) to produce free-floating sections.
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Description
Beta-Galactosidase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Beta Galactosidase [E.coli]. Cross reactivity against Beta Galactosidase from other tissues and species may occur but have not been specifically determined. Very low background staining has been reported in various assays.
Immunogen
Full length native Beta Galactosidase isolated from E.coli
Hao G. Nguyen, Guangyao Yu, Maria Makitalo, Dan Yang, Hou-Xiang Xie, Matthew R. Jones, and Katya Ravid (2005) Conditional overexpression of transgenes in megakaryocytes and platelets in vivo. Blood 2005 106:1559-1564; doi: https://doi.org/10.1182/blood-2005-02-0638