ELISA, Western Blot. This antibody is suitable for western blotting, immunoprecipitation, and ELISA. This antibody detects mouse Mcl-1 and is not expected to cross react with the human sequence. Cross reactivity with Mcl-1 from other sources is unknown. This antibody is highly specific, showing no bands in null cell lysates from Mcl-1 knockout mice even when grossly over-exposed. Mouse Mcl-1 (myeloid cell leukemia sequence 1) is composed of 331 amino acids and is reported to be 35.2 kDa in size. The human ortholog consists of 351 amino acids and is reported to be 37.3 kDa in size.
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Description
This affinity-purified antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads followed by cross adsorption to remove any unwanted reactivity. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Immunogen
This affinity purified antibody was purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Mcl-1 conjugated to Keyhole Limpet Hemocyanin (KLH).
Other Names
rabbit anti-Mcl-1 antibody, Mcl1, Mcl 1, Bcl 2 related protein EAT/mcl1 antibody, Bcl2 related antibody, EAT antibody, Induced myeloid leukemia cell differentiation protein Mcl-1 antibody
Rutherford C, Childs S, Ohotski J, McGlynn L, Riddick M, MacFarlane S, Tasker D, Pyne S, Pyne NJ, Edwards J, Palmer TM. (2013) Regulation of cell survival by sphingosine-1-phosphate receptor S1P1 via reciprocal ERK-dependent suppression of Bim and PI-3-kinase/protein kinase C-mediated upregulation of Mcl-1. Cell Death Dis. 2013 Nov 21;4:e927. doi: 10.1038/cddis.2013.455.Perciavalle RM, Stewart DP, Koss B, Lynch J, Milasta S, Bathina M, Temirov J, Cleland MM, Pelletier S, Schuetz JD, Youle RJ, Green DR, Opferman JT. (2012) Anti-apoptotic MCL-1 localizes to the mitochondrial matrix and couples mitochondrial fusion to respiration. Nat Cell Biol. 2012 Apr 29;14(6):575-83. doi: 10.1038/ncb2488. Omari S, Waters M, Naranian T, Kim K, Perumalsamy AL, Chi M, Greenblatt E, Moley KH, Opferman JT, Jurisicova A. (2015) Mcl-1 is a key regulator of the ovarian reserve. Cell Death Dis. 2015 May 7;6:e1755. doi: 10.1038/cddis.2015.95.