ELISA, Flow Cytometry, Immunohistochemistry, Western Blot. This monoclonal antibody contains TBS/1% BSA and is suitable for ELISA, immunohistochemistry, flow cytometry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 23 kDa in size corresponding to CD3 protein by western blotting in the appropriate cell lysate or extract. Jurkat cell lysates can be used as a control for western and flow. For immunohistochemistry, samples should be formalin fixed and paraffin embedded. Deparaffinize slides using xylene or xylene alternatives and graded alcohols. Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min. CD3 is a membrane protein. Tonsil tissue can be used as a positive control.
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Description
CD3 Antibody is clarified tissue culture supernatant. The antibody is specific for human CD3 protein. Cross-reactivity is known to occur with baboon, monkey, horse, dog and cat sources of CD3. Cross reactivity with CD3 protein from other sources has not been determined.
Immunogen
Anti-CD3 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues 156-168 of the epsilon chain of human CD3 protein. The hybridoma was produced by fusing New Zealand White rabbit splenocytes and myeloma cells using conventional technology.