FLISA, Flow Cytometry, Immunohistochemistry, IF Microscopy, Western Blot. Mouse IgG1 FITC conjugated secondary antibody reagents are ideal for Flow Cytometry, Immunohistochemistry and Immunofluorescence Microscopy as well as other antibody detection methods. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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Description
Anti-Mouse IgG1 FITC conjugated secondary antibody prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG1 coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.