The microtiter plate provided in this kit has been pre-coated with an antibody specific to COL IV. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for COL IV and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain COL IV , biotin-conjugated antibody an