ELISA, Immunohistochemistry, Western Blot. This affinity purified antibody is suitable for use in ELISA and western blotting assays. This reagent is particularly useful to differentiate polymorphic forms of NAG-1 protein present in human serum samples. This antibody is useful in dual antibody immunometric assays (EIA). Specific conditions for reactivity should be optimized by the end user.
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Description
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody specifically reacts with a D variant sequence of human NAG-1 protein from human tissues. A BLAST analysis was used to suggest partial reactivity with NAG-1 from chimpanzee and macaque based on a 92% homology. Cross-reactivity with NAG-1 from other sources has not been determined.
Immunogen
This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminal end of human NAG-1 protein. A residue of cysteine was added to facilitate coupling to KLH.