Glycerol [Glycerin or Glycerine, C3H5(OH)3] is widely used in foods, beverages and pharmaceutical formulations. It is also a main by- product of biodiesel production. Simple, direct and automation-ready procedures for measuring glycerol concentrations find wide Applications:. Glycerol assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at lem/ex=585/530nm is directly proportional to glycerol concentration in the sample. Key Features: Sensitive and accurate. Use as little as 10ul samples. Linear detection range in 96-well plate: 10 to 1000uM (92 ug/dL to 9.2 mg/dL) glycerol for Colorimetric Assay:s and 2 to 50uM for Fluorometric Assay:s. Simple and convenient. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature, compatible for HTS assays. Improved reagent stability. The optimized fo