ELISA, Western Blot. This protein A purified monoclonal antibody against human Hsp27 has been tested for use in immunoblotting, immunoprecipitation, ELISA, immunohistochemistry and immunocytochemistry. The antibody recognizes a 27 kDa band corresponding to hsp27 in cell lysates from breast carcinoma. Both frozen sections and paraffin embedded material can be used for immunocytochemistry and immunohistochemistry.
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Description
This protein A purified mouse monoclonal antibody reacts specifically with HSP27 in human tissues and cell lines. MCF-7 cells are recommended as a positive control. Cross reactivity with hsp27 from other mammalian sources is likely. No cross reactivity occurs with HSP70, HSP90 or HSP104.
Immunogen
This HSP27 monoclonal antibody was produced by repeated immunizations with a prokaryotic recombinant protein corresponding to the full length human hsp27 protein.
Other Names
mouse anti-HSP27 antibody, mouse anti-Heat shock protein beta-1 antibody, HspB1 antibody, Heat shock 27 kDa protein antibody, Stress-responsive protein 27 antibody, SRP27 antibody, Estrogen-regulated 24 kDa protein antibody, 28 kDa heat shock protein antibody
Ines Moretti-Rojas, et. al. (1988) A cDNA for the estradiol-regulated 24K protein: control of mRNA levels in MCF-7 cells. Breast Cancer Res. and Treat., 11: 155-163.McGuire, S.E. et. al. (1989) Chromasomal Assignment of Human 27-kDa Heat Shock Protein Gene Family. Somatic Cell and Molecular Genetics, 15: 167-171.Edwards, D.P., et. al. (1981) Estradiol stimulates synthesis of a major intracellular protein in a human breast cancer cell line (MCF-7). Breast Cancer Res. Treat., 1: 209-223.