human, bovine, mouse, rat, C. elegans, dog, chicken, Drosophila, carp, guinea pig, hamster, monkey, porcine, rabbit, sheep
Isotype
IgG1
Format
Protein G purified
Size
100 μg
Concentration
1.0 mg/ml
Applications
Immunohistochemistry, IF Microscopy, Western Blot. This Protein G purified antibody has been tested for use in ELISA, immunofluorescence microscopy, western blotting, FACS, immunoelectron microscopy, immunohistochemistry and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 70 kDa in size corresponding to Hsp70 by western blotting in the appropriate cell lysate or extract. In general, a 1:1,000 dilution is suggested for most applications and is suitable to detect Hsp70 in 20 μg of heat shocked HeLa cell lysate by western blotting.
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Description
This Protein G purified monoclonal antibody reacts with human and mouse Hsp70 protein. A BLAST analysis was used to suggest cross-reactivity with Hsp70 from human, bovine, mouse, rat, C.elegans, dog, chicken, Drosophila, carp, guinea pig, hamster, monkey, pig, rabbit and sheep sources based on 100% homology with the immunizing sequence. No cross-reactivity occurs with hsc70 (hsp73). Cross-reactivity with Hsp70 from other sources has not been determined.
Immunogen
Hsp70 monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with a synthetic peptide from the region of amino acid residues 436-503 of human Hsp70 protein.
Other Names
Heat shock 70 kDa protein 1 antibody, heat shock 70kDa protein 1A antibody, Heat shock 70kDa protein 1B antibody, Heat shock induced protein antibody, heat shock protein 70 antibody, HSP70 1 antibody