ELISA, Immunohistochemistry, Western Blot. Anti-Rabbit IgG peroxidase conjugated antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
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Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Immunogen
Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in donkey.
Thomas S Klaric, Paul Q Thomas, Mirella Dottori, Wai Khay Leong, Simon A Koblar, Martin D Lewis. (2014) A reduction in Npas4 expression results in delayed neural differentiation of mouse embryonic stem cells. Stem Cell Research & Therapy20145:64. doi.org/10.1186/scrt453.