ELISA, Immunohistochemistry, Western Blot. This affinity purified Stat3 antibody has been tested for use in ELISA, IHC, and Western Blot. Expect a band approximately 88.1 kDa in size corresponding to phosphorylated Stat3 protein by western blotting in the appropriate stimulated tissue or cell lysateor extract. Specific conditions for reactivity should be optimized by the enduser.
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Description
Anti-STAT3 antibody is an affinity-purified antibody and is directed against the phosphorylated form of human Stat3 protein at the Y705. Anti-Stat3 antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Anti-Stat3 antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated Stat3 protein is minimal by ELISA and western blot. A BLAST analysis was used to suggest cross reactivity with Stat3 antibodies from human, mouse, rat, and pig based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Immunogen
This affinity purified STAT3 antibody was prepared by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids surrounding tyrosine 705 of human Stat3.
Other Names
rabbit anti-STAT3 pY705 antibody, Signal transducer and activator of transcription 3, Acute-phase response factor, APRF, STAT3 phospho Y705, STAT3 (pTry705)