ELISA, Immunohistochemistry, Western Blot. This monoclonal antibody is suitable for ELISA, immunohistochemistry and western blotting. Expect a band approximately 106 kDa in size corresponding to TRPC6 protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required. Strong staining was observed in adrenal, Purkinje neurons, cortical neurons, heart, ganglion cells, renal tubules, Sertoli cells, hepatocytes, skeletal muscle, exocrine pancreas, and germinal centers of lymphoid follicles. Moderate staining was observed in colon epithelium and plasma cells, B-lymphocytes, and parafollicular cells of the thyroid. Faint staining was seen in respiratory epithelium. Prostate and placenta were negative for staining. The antibody produced minimal to no background staining and appeared very specific at 2.5 μg/mL.
Reviews / Ratings
If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY.
Description
This product was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human TRPC6 protein. A BLAST analysis was used to suggest cross-reactivity with TRPC6 from chimpanzee based on 100% homology with the immunizing sequence. Cross-reactivity with TRPC6 from other sources has not been determined.
Immunogen
This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human TRPC6 protein.
Other Names
mouse anti-TRPC6 Antibody, TRPC 6, TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6