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| Antigenic Specificity | Sheep IgG |
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| Clone | eB270 |
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| Host Species | Mouse |
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| Reactive Species | sheep |
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| Isotype | n/a |
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| Format | peroxidase (HRP) conjugate |
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| Size | 50 μL |
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| Concentration | 1000X (see application note, titrated reagent enough for 20 blots) |
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| Applications | Western Blot. Sheep IgG TrueBlot? is provided as 1000X solution. In order to conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mls). If used conservatively at 2.5mls/blot, cat. 18-8815-31 (50μL) will yield enough reagent for 20 blots.Note that there are three key procedural considerations:1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands.2. Immunoprecipitate should be completely reduced.3. Milk should be used as the blocking protein for the immunoblot. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | Sheep TrueBlot? Antibody Peroxidase Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum. Reactivity is observed against native Sheep IgG by both Western blot and ELISA. |
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| Immunogen | n/a |
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| Other Names | HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting |
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| Gene, Accession # | n/a |
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| Catalog # | 18-8815-31 |
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| Price | |
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| Order / More Info | Sheep IgG Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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