Purity: > 95% by SDS - PAGE Format: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl . NAD-dependent deacetylase sirtuin-3, mitochondrial, also known as SIRT3, belongs to the sirtuin family of proteins. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have a range of molecular functions and have emerged as important proteins in aging, stress resistance and metabolic regulation. SIRT3 exhibits NAD+-dependent deacetylase activity in the mitochondria. Over-expression of SIRT3 results in increased levels of the mitochondrial uncoupling protein 1. SIRT3 protein levels are also elevated in certain breast cancers. Recombinant human SIRT3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.