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| Antigenic Specificity | DYKDDDDK |
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| Clone | 29E4.G7 |
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| Host Species | Mouse |
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| Reactive Species | n/a |
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| Isotype | IgG2a kappa |
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| Format | DyLight? 549 conjugate |
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| Size | 100 μg |
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| Concentration | 1.0 mg/mL |
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| Applications | ELISA, FLISA, IF Microscopy, Western Blot. The emission spectra for this DyLight? conjugate match the principle output wavelengths of most common fluorescence instrumentation. This antibody is optimally suited for monitoring the expression of FLAG? tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG? epitope. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This antibody is directed against the FLAG? epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the FLAG? epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. |
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| Immunogen | This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG? epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling. |
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| Other Names | mouse anti-FLAG? tag DyLight?549 conjugation, Enterokinase Cleavage Site (ECS), DyLight?549 conjugated mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys |
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| Gene, Accession # | n/a |
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| Catalog # | 200-342-383 |
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| Price | |
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| Order / More Info | DYKDDDDK Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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