ELISA, Immunohistochemistry, Western Blot. Anti-Human Transferrin Peroxidase is designed for Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates.
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Description
This reagent was purified from pooled rabbit sera obtained from rabbits immunized with Human Transferrin. Purification was by Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification. The purified antibody was then conjugated to horseradish peroxidase and subsequently purified followed by exhaustive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, purified Human Transferrin and Human Serum.