ELISA, Immunohistochemistry, Western Blot. Anti-Huntingtin pS421 antibody has been tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect bands at approximately 350 kDa and 200 kDa in size corresponding to full-length Huntingtin protein and truncated (hypothetical) Huntingtin protein, respectively, by western blotting in the appropriate cell lysate or extract. This antibody is specific for the phosphorylated form of Huntingtin protein at the pS421 residue. The identity of lower molecular bands ~130 kDa is not known.
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Description
Anti-Huntingtin pS421 is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human Huntingtin protein at the pS421 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Huntingtin homologues from chimpanzee, pig and chicken. Cross reactivity with Huntingtin protein homologues from mouse and rat may also occur as sequence homology varies by one amino acid residue in this sequence. Reactivity with Huntingtin protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.
Immunogen
Huntingtin pS421 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 416-424 of Human huntingtin protein.