ELISA, Immunoprecipitation, Western Blot. This affinity-purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38 kDa in size corresponding to Hus1B protein by western blotting in the appropriate cell lysate or extract.
Reviews / Ratings
If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY.
Description
This affinity-purified antibody is directed against human Hus1B protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis indicates 100% homology of the immunizing sequence with Hus1B protein from human. Partial reactivity with mouse Hus1B is not likely based on low (~64%, 9/14) sequence homology. Reactivity against homologues from other sources is not known.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 104-117 of human Hus1B protein.