ELISA, Immunohistochemistry, Western Blot. Anti-TIMP4 antibody has been tested for use in ELISA, Native - Western, and IHC (P). SDS-PAGE - Western Blot not useful for endogenous detection. Specific conditions for reactivity should be optimized by the end user.
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Description
Anti-TIMP4 was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above. TIMP4 antibody is specific for human TIMP4 protein. This antibody reacts with human TIMP4 in tissue sections of formalin-fixed, paraffin-embedded In ELISAs and Native-PAGE, this antibody does not cross-react with recombinant human TIMP-1, -2 or -3. A BLAST analysis was used to suggest cross-reactivity with TIMP4 from human and mouse sources based on the immunizing sequence. Cross-reactivity with TIMP-4 from other sources has not been determined.
Immunogen
Anti-TIMP4 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with full legnth human TIMP4 protein followed by hybridoma development.
Other Names
mouse anti-TIMP4 antibody, Tissue inhibitor of metalloproteinase-4, Metalloproteinase inhibitor 4, TIMP-4, TIMP 4, TIMP4 antibody, anti-TIMP-4 antibody
Donover PS., Wojciechowski BS., Thirumanran R., Zemba-Palko V., Prendergast GC., Wallon UM. (2010) Development of a Monoclonal Antibody That Specifically Detects Tissue Inhibitor of Metalloproteinase-4 (TIMP-4) in Formalin-Fixed, Paraffin-Embedded Human Tissues. Journal of Cellular Biochemistry 110:1255-1261.