ELISA, Western Blot. Anti-TLR4 affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 96 kDa in size corresponding to TLR4 protein in overexpressed lysate by western blotting in the appropriate cell lysate or extract.
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Description
This affinity purified antibody is directed against human TLR4 protein. The product was purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest reactivity with TLR4 protein from human based on 100% homology with the immunizing sequence. The analysis also shows 100% cross-reactivity with gorilla and bonobo, and canine species. TLR4 (Toll-Like Receptor 4). Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kB. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis. Mutations in this gene have been associated with differences in LPS responsiveness, macular degeneration, and pertussis. Anti-TLR4 Antibody is useful for researchers interested in transcription factor research, cytokine research, MIF action through Endocytic Pathway, and rheumatoid arthritis.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the n-terminus of human TLR4 protein.