IF Microscopy, Western Blot. Anti-HDEL Antibody is recommended for use in WB and IF microscopy. Expect a band approximately ~78kDa corresponding to specific lysates. Specific conditions for reactivity should be optimized by the end user.
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Description
Anti-HDEL Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with HDEL from Drosophilia, yeast, Saccharomyce, and Plants (Barnyard grass, beet, cotton, mung bean, sorghum, wheat) based on 100% homology with the immunizing sequence. Cross-reactivity with HDEL from other sources has not been determined. Heat Shock and trafficking research.
Immunogen
HDEL Antibody was produced in mice by repeated immunizations raised against a synthetic HDEL synthetic peptide corresponding to the C-terminus of yeast Bip.
Other Names
H-D-E-L, endoplasmic reticulum, luminal ER protein retention