ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot. This monoclonal antibody can be used for hemagglutination inhibition (HI) assays to provide antigenic characterization of the influenza A viruses of the H5 HA subtype. This monoclonal antibody is suitable for virus neutralization assays (in cell culture and in embryonated chicken eggs), ELISA, immunoprecipitation, immunohistochemistry and western blotting.
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Description
This product was clarified from mouse ascitic fluid and is specific for H5 hemagglutinin (HA) protein of influenza A virus [strain A/Vietnam/1203/04 (H5N1)]. VN04-8 monoclonal antibody did not cross-react with influenza viruses of other HA subtypes. This monoclonal antibody reacted with H5N1 influenza virus representatives of different clades and subclades of the H5 HA subtype.
Immunogen
This monoclonal antibody was produced by intraperitoneal immunization of BALB/c mice with concentrated purified virus preparation containing hemagglutinin (HA) protein of influenza A virus [strain A/Vietnam/1203/04 (H5N1)] using the modification of the method described by Kohler and Milstein. Each mouse received two immunizations of 15 μg HA with incomplete Freund's adjuvant, administered 3 week apart.
Govorkova, E.A., et al. (2006) Immunization with Reverse-Genetics-Produced H5N1 Influenza Vaccine Protects Ferrets against Homologous and Heterologous Challenge. J. Infect. Dis. 194:159-167.Hoffmann, E., et al. (2005) Role of Specific Hemagglutinin Amino Acids in the Immunogenicity and Protection of H5N1 Influenza Virus Vaccines. Proc. Natl. Acad. Sci. 102(36): 12915-12920.ML Reed, HL Yen, RM DuBois, OA Bridges, R Salomon, RG Webster, CJ Russell. (2009). Amino Acid Residues in the Fusion Peptide Pocket Regulate the pH of Activation of the H5N1 Influenza Virus Hemagglutinin Protein. J Virol. 2009 April; 83(8): 3568:3580. BJ Hanson, A Boon, APC Lim, A Webb, EE Ooi, RJ Webby. (2006). Passive immunoprophylaxis and therapy with humanized monoclonal antibody specific for influenza A H5 hemagglutinin in mice. Respiratory Research 2006, 7:126.