ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot. Hemagglutinin of A/Vietnam/1203/04 Influenza Virus (VN04-10) monoclonal antibody can be used for hemagglutination inhibition (HI) assays to provide antigenic characterization of the influenza A viruses of the H5 HA subtype. This monoclonal antibody is suitable for virus neutralization assays (in cell culture and in embryonated chicken eggs), ELISA, immunoprecipitation, immunohistochemistry and western blotting.
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Description
H5N1 Antibody was purified from tissue culture supernatant fluid by Protein A chromatography and is specific for H5 hemagglutinin (HA) protein of influenza A virus [strain A/Vietnam/1203/04 (H5N1)]. VN04-10 monoclonal antibody did not cross-react with influenza viruses of other HA subtypes. This monoclonal antibody reacted with H5N1 influenza viruses representatives of different clades and subclades of the H5 HA subtype.
Immunogen
Hemagglutinin of A/Vietnam/1203/04 Influenza Virus (VN04-10) monoclonal antibody was produced by intraperitoneal immunization of BALB/c mice with concentrated purified virus preparation containing hemagglutinin (HA) protein of influenza A virus [strain A/Vietnam/1203/04 (H5N1)] using the modification of the method described by Kohler and Milstein. Each mouse received two immunizations of 15 μg HA with incomplete Freund's adjuvant, administered 3 week apart.
Govorkova, E.A., et al. (2006) Immunization with Reverse-Genetics-Produced H5N1 Influenza Vaccine Protects Ferrets against Homologous and Heterologous Challenge. J. Infect. Dis. 194:159-167.Hoffmann, E., et al. (2005) Role of Specific Hemagglutinin Amino Acids in the Immunogenicity and Protection of H5N1 Influenza Virus Vaccines. Proc. Natl. Acad. Sci. 102(36): 12915-12920.ML Reed, HL Yen, RM DuBois, OA Bridges, R Salomon, RG Webster, CJ Russell. (2009). Amino Acid Residues in the Fusion Peptide Pocket Regulate the pH of Activation of the H5N1 Influenza Virus Hemagglutinin Protein. J Virol. 2009 April; 83(8): 3568:3580. BJ Hanson, A Boon, APC Lim, A Webb, EE Ooi, RJ Webby. (2006). Passive immunoprophylaxis and therapy with humanized monoclonal antibody specific for influenza A H5 hemagglutinin in mice. Respiratory Research 2006, 7:126.