Titrated value sufficient to run approximately 10 mini blots.
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot. Anti-NMDA NR2B subunit (Rabbit) antibody is suitable for use in Western Blotting, Immunoprecipitation and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band or approximately 180 kDa in size corresponding to the NR2B subunit of the NMDA receptor in the appropriate cell lysate or extract. This antibody is lyophilized from 5 mM ammonium bicarbonate. Reconstitute material with 50 μL PBS pH 7.4 before use.
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Description
Anti-NMDA 2B antibody is directed against NMDA receptor protein. The antibody was affinity purified from monospecific antiserum by immunoaffinity purification. The antibody recognizes human, mouse and rat forms of the NR2B subunits of NMDAR. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody. No reactivity towards the NR2A and NR2C subunits was seen. This antibody is lyophilized from 5 mM ammonium bicarbonate.
Immunogen
Anti-NMDA NR2B Antibody was produced by repeated immunizations with a fusion protein from the C-terminal region NR2B subunit.