ELISA, Immunohistochemistry, Western Blot. CTCF antibody is suitable for ELISA, Immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 82.8 kDa or 150 kDa in size corresponding to CTCF by western blotting in the appropriate cell lysate or extract.
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Description
Anti-CTCF was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with endogenous CTCF protein. A BLAST analysis was used to suggest reactivity with CTCF from human, mouse, horse, bovine, panda, rabbit, Danio, and chicken based on a 100% homology with the immunizing sequence. Cross-reactivity with CTCF from other sources has not been determined.
Immunogen
CTCF affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of CTCF protein.