ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot. Anti-Osteopontin is suitable for western blotting, immunohistochemistry (formalin-fixed paraffin-embedded sections) and ELISA. The antibody exclusively recognizes C-terminal fragments of both thrombin and MMP-cleaved OPN. A 1:1000 dilution will detect strongly approximately 250 ng of OPN protein on a blot. No pretreatment is required for IHC when formalin-fixed paraffin-embedded tissue is stained.
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Description
Osteopontin is directed against human osteopontin. The antibody recognizes the full-length osteopontin protein (which runs at 66 kDa on westerns), as well as the C-terminal fragments of both thrombin and MMP-cleaved OPN. The 32 kDa MMP-cleaved C-fragment is recognized, but not the 40 kDa N-terminal fragment. Reactivity is reported to occur with osteopontin from swine, dog, mouse and rat.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, from the human osteopontin protein, conjugated to KLH using maleimide.
Other Names
rabbit anti-Osteopontin antibody, Bone sialoprotein 1, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Nephropontin, Uropontin, SPP1, BNSP, OPN
Kim SJ, Shin TK. Immunohistochemical study of osteopontin in boar testis. J Vet Sci. 2007 June; 8(2): 107-110. Published online 2007 June 30. doi: 10.4142/jvs.2007.8.2.107 PMCID: PMC2872706Robertson BW, Bonsal L, Chellaiah MA. Regulation of Erk1/2 activation by osteopontin in PC3 human prostate cancer cells. Mol. Cancer. 2010;9:260. Robertson BW, Chellaiah MA. Osteopontin induces beta-catenin signaling through activation of Akt in prostate cancer cells. Exp. Cell Res. 2010 Jan 1;316(1):1-11.Cherepanova O, Gomez D, Shankman L, Swiatlowska P, Williams J, Sarmento O, Alencar G, Hess D, Bevard M, Greene E, Murgai M, Turner S, Geng Y, Bekiranov S, Connelly J, Tomilin A, Owens G. (2016) Activation of the pluripotency factor OCT4 in smooth muscle cells is atheroprotective. Nature Medicine volume 22, pages 657-665.