ELISA, Chip, Immunohistochemistry, Immunoprecipitation, Western Blot. This antibody is suitable for ChIP, flow cytometry, immunohistochemistry, immunoblotting and immunoprecipitation. p53 is the most commonly mutated gene in spontaneously occurring human cancers. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dental fibroblasts following mild ultraviolet irradiation. This antibody reacts with an N-terminal epitope of the 53 kD gene product and this epitope is not destroyed by formalin-fixation and routine paraffin embedding. Microwaving is needed for optimal staining.
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Description
This protein A purified mouse monoclonal antibody reacts specifically with p53 in human tissues and cell lines. The antibody recognizes a 53 kDa band corresponding to p53. Cross reactivity with p53 from other sources has not been determined.
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p53 protein.
Gurney, E. G. et al (1980) Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct sublcasses of large T antigen and for similarities among nonviral T antigens. J Virol, 34(3):752-63. Hollstein, M, et al. (1991) p53 mutations in human cancers. Science, 253: 49-53.Lane, D.P. (1992) p53, guardian of the genome. Nature, 358(6381):15-16