ELISA, Western Blot. This affinity-purified antibody has been tested for use in ELISA. Specific conditions for reactivity should be optimized by the end user.
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Description
This affinity purified antibody is directed against human PPAR gamma protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest broad reactivity with this protein from several sources. Expect reactivity against human (g1 and g2), mouse (g1 and g2), rat, squirrel, orangutan, duck (g1), macaque (g1-g7), boar (g1a-1d), mink, guinea pig, rabbit (g1 and g3), dog and hamster sources based on 100% homology for the immunogen sequence. Cross reactivity with PPAR gamma homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 255 -268 of human PPAR gamma isoform 1.
Other Names
rabbit anti-PPAR gamma 1 and 2 antibody, Peroxisome proliferator-activated receptor gamma, Nuclear receptor subfamily 1 group C member 3, NR1C3, PPARG, PPAR gamma, PPARG1 antibody, PPARG2 antibody
Bruedigam C, Koedam M, Chiba H, Eijken M, van Leeuwen JP. (2008) Evidence for multiple peroxisome proliferator-activated receptor gamma transcripts in bone: fine-tuning by hormonal regulation and mRNA stability. FEBS Lett. 2008 May 14;582(11):1618-24. doi: 10.1016/j.febslet.2008.04.012. Epub 2008 Apr 22.