ELISA, Immunohistochemistry, Western Blot. This antibody reacts with human Cul1 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated product and protein from cell lysates (using HeLa or NIH-3T3). Do not IP in the presence of NP-40, but rather use 0.1% SDS. An 89.6 kDa band corresponding to human Cul1 is detected. Most cell lines expressing Cul1 can be used as a positive control. Researchers should determine optimal titers for other applications.
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Description
This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 1. Cross reactivity is expected against mouse Cul1 based on sequence homology.
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 766-776 of Human Cul1 (C-terminus) coupled to KLH.
Michel,J.J. and Xiong,Y. (1998) Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A. Cell Growth Differ. 9 (6), 435-449.Kipreos,E.T., Lander,L.E., Wing,J.P., He,W.W. and Hedgecock,E.M. (1996) cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family. Cell 85 (6), 829-839.Ohta,T., Michel,J.J., Schottelius,A.J. and Xiong,Y. (1999) ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Mol. Cell 3 (4), 535-541.