ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot. This antibody reacts with human Cul2 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated product and protein from cell lysates (using HeLa or NIH-3T3). An 86.9 kDa band corresponding to human Cul2 is detected. Most cell lines expressing Cul2 can be used as a positive control. Researchers should determine optimal titers for other applications.
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Description
Antibody is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 2. Cross reactivity with Cul2 from other sources has not been determined.
Immunogen
Anti-Cul2 was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 733-745 of Human Cul2 (C-terminus) coupled to KLH.
Feng, H., Zhong, W., Punkosdy, G., Gu, S., Zhou, L., Seabolt,. E.K. and Kipreos, E.T. (1999) CUL-2 is required for the G1-to S-phase transition and mitotic chromosome condensation in Caenorhabditis elegans. Nat Cell Bio 1, 486-492. Kipreos,E.T., Lander,L.E., Wing,J.P., He,W.W. and Hedgecock,E.M. (1996) cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family. Cell 85 (6), 829-839.Michel,J.J. and Xiong,Y. (1998) Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A. Cell Growth Differ. 9 (6), 435-449.