ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot. This antibody reacts with human Cul3 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated product and protein from cell lysates (using HeLa or NIH-3T3). An 88.9 kDa band corresponding to human Cul3 is detected. Most cell lines expressing Cul3 can be used as a positive control. Researchers should determine optimal titers for other applications.
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Description
Anti-Cul3 antibody is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 3. Cross reactivity is expected against mouse Cul3 based on sequence homology. Cross reactivity with other human cullins is unlikely based on sequence homology.
Immunogen
Cul3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1-12 of Human Cul3 (N-terminus) coupled to KLH.
Furukawa, M., He, Y.J., Borchers, C., and Xiong, Y. (2003) Targeting of ubiquitination by BTB-Cullin3-Roc1 ubiquitin ligases. Nat Cell Biol. 5, 1001-1007. Kipreos,E.T., Lander,L.E., Wing,J.P., He,W.W. and Hedgecock,E.M. (1996) cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family. Cell 85 (6), 829-839.Michel,J.J. and Xiong,Y. (1998) Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A. Cell Growth Differ. 9 (6), 435-449.