ELISA, Immunoprecipitation, Western Blot. This antibody has been tested for use in ELISA and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kDa in size corresponding to Cybr by western blotting after IP in the appropriate cell lysate or extract. To date, this antibody has not shown direct detection of endogenous or over-expressed Cybr protein, but is capable of immunoprecipitating the antigen from lysates. It is possible, but has not yet been confirmed, that this antibody recognizes a conformational epitope.
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Description
This Cybr antibody is directed against mouse Cybr. The product is delipidated and defibrinated antiserum. A BLAST analysis was used to suggest cross-reactivity with Cybr from mouse and rat based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Immunogen
PSCDBP antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region in the C-terminal portion of mouse Cybr.
Other Names
rabbit anti-Cybr antibody, Cytohesin binder and regulator antibody, Cytohesin-binding protein HE antibody, Cytohesin-interacting protein antibody, Pleckstrin Homology Sec7 and Coiled-Coil Domains Binding Protein antibody, B31 antibody, PSCDBP antibody