ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot. This Protein A purified antibody has been tested for use in ELISA, immunohistochemistry western blot, and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 59 kDa in size corresponding to Cyclin L1a by western blotting in the appropriate cell lysate or extract.
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Description
The product was purified from monospecific antiserum by Protein A chromatography. This antibody is specific for human Cyclin L1a and shows negligible reactivity to Cyclin L1b or Cyclin L2 isoforms. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with Cyclin L1a protein from dog, rat, mouse and chicken is expected, as this sequence shows 87-96% homology with the protein from these sources. Cross reactivity with Cyclin L1a homologues from other sources has not been determined.
Immunogen
This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic polypeptide corresponding to amino acids 314-369 of Human Cyclin L1a protein.
Other Names
rabbit anti-Cyclin L1 antibody, Cyclin-L, Cyclin L beta, Cyclin L gamma, CCNL1
Loyera, P., Trembleya, J.H., Katonac, R., Kidd, V.J. and Lahtia, J.M. (2005) Role of CDK/cyclin complexes in transcription and RNA splicing. Cell. Signal. 17; 1033 - 1051