ELISA, Immunoprecipitation, Western Blot. This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 58 kDa in size corresponding to Cyclin L2a by western blotting in the appropriate cell lysate or extract. Although not tested, this antibody is likely functional in immunohistochemistry, immunofluorescence, and immunoprecipitation.
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Description
This product is an affinity purified antibody produced by immunoaffinity chromatography using polypeptide coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This antibody is specific for human Cyclin L2a and shows negligible reactivity to Cyclin L2b or Cyclin L1 isoforms. A BLAST analysis was used to suggest reactivity with this protein from mouse based on 100% homology for the immunogen sequence. Cross reactivity with Cyclin L2a protein from rat and chicken (called Cyclin S) is expected, as this sequence shows ~ 80% homology with the protein from these sources. Cross reactivity with Cyclin L2a homologues from other sources has not been determined.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic polypeptide corresponding to amino acids 309-384 of Human Cyclin L2a protein.
Loyera, P., Trembleya, J.H., Katonac, R., Kidd, V.J. and Lahtia, J.M. (2005) Role of CDK/cyclin complexes in transcription and RNA splicing. Cell. Signal. 17; 1033 - 1051.