ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot. This Protein A purified antibody has been tested for use in ELISA, western blot, immunohistochemistry, and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 25 kDa to 35 kDa in size corresponding to Cyclin L1b and Cyclin L2b by western blotting in the appropriate cell lysate or extract.
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Description
The product was purified from monospecific antiserum by Protein A chromatography. This antibody will react with all forms of Cyclin L b (cyclin L1b and cyclin L2b). Cross reactivity with Cyclin L beta proteins from other sources is unknown.
Immunogen
This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic construct consisting of full length Human Cyclin L1b protein.
Loyera, P., Trembleya, J.H., Katonac, R., Kidd, V.J. and Lahtia, J.M. (2005) Role of CDK/cyclin complexes in transcription and RNA splicing. Cell. Signal. 17; 1033 - 1051.