ELISA, Immunohistochemistry, Western Blot. This product has been assayed against 1.0 ug of Horse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:40,000 to 1:130,000 of the reconstitution concentration is suggested for this product.
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Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Horse IgG, Horse IgG F(c) and Horse Serum. No reaction was observed against Horse IgG F(ab).
Immunogen
Horse IgG F(c) fragment
Other Names
Goat Anti-Horse IgG F(c) Antibody Biotin Conjugated, Goat Anti Horse IgG Fc Fragment Antibody Biotin Conjugated, Goat Anti Horse IgG Fc Antibody Biotin Conjugated