ELISA, Immunohistochemistry, IF Microscopy, Western Blot. Anti-HDAC-1 Antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Specific nuclear staining is observed by IHC. Expect bands at 65 kDa in size corresponding to HDAC-1 by western blotting in the appropriate cell lysate or extract.
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Description
Anti-HDAC-1 antibody is directed against human HDAC-1 protein. HDAC-1 antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat and chimpanzee sources based on 100% homology for the immunogen sequence. Cross reactivity may occur with HDAC-1 from bovine (82% homology) and chicken (80% homology) sources. Cross reactivity with HDAC-1 homologues from other sources has not been determined.
Immunogen
Anti-HDAC-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 466-482 of Human HDAC-1.
Nohata N, Hanazawa T, Kinoshita T, Inamine A, Kikkawa N, Itesako T, Yoshino H, Enokida H, Nakagawa M, Okamoto Y, Seki N. (2013) Tumour-suppressive microRNA-874 contributes to cell proliferation through targeting of histone deacetylase 1 in head and neck squamous cell carcinoma. Br J Cancer. 2013 Apr 30;108(8):1648-58. doi: 10.1038/bjc.2013.122. Epub 2013 Apr 4.