Anti-PARP1 (internal) antibody with the matched secondary has been validated by western blotting and nanoimmunoassay (NIA). Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 113 kDa in size corresponding to PARP-1 by western blotting in the appropriate cell lysate or extract. PARP1 (internal) Control Protein analysis by SDS-PAGE resulted in a band at a MW ~19 kDa and was estimated to be greater than 95% pure by Coomassie staining.
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Description
PARP1 (internal) was purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads. This antibody is specific for human PARP1 protein. No cross reactivity detected towards other PARP members when using siRNAs against 18 PARP family members. Cross-reactivity with PARP1 from other sources has not been determined.PARP1 (internal) is a c-terminus His-Tag recombinant protein expressed in E.coli corresponding to a fragment of the human PARP1 autocatalytic domain. RABBIT IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Immunogen
PARP1 (internal) purified antibody was prepared from whole rabbit serum produced by repeated immunizations with c-terminus region of human PARP1 autocatalytic domain recombinant protein.Anti-Rabbit IgG HRP secondary antibody was produced by repeated immunizations with Rabbit IgG whole molecule.